Database : HANSEN Search on : ANALISE DE SEQUENCIA DE DNA [Subject descriptor] References found : 22 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 17564 Author: Kramme, Stefanie; Bretzel, Gisela; Panning, Marcus; Kawuma, Joseph; Drosten, Christian Title: Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR ..- Source: s.l; s.n; 2004. 5 p. tab, graf. Abstract: Real-time PCR technology has improved molecular diagnostics of many pathogens, but no such test is available for Mycobacterium leprae. In this report we describe the establishment and the pre-clinical evaluation of such an assay. The test achieved a theoretical analytical sensitivity limit of 194 M. leprae cells per skin biopsy specimen and facilitated quantification of mycobacteria in tissue over a range of 54-54,000,000 cells per sample. In punch skin biopsies from 39 untreated Ugandan patients with newly diagnosed leprosy, the clinical diagnosis could be confirmed in 88.9% of multibacillary and 33.3% of paucibacillary (microscopically negative) patients. Real-time detection thus did not increase the clinical sensitivity of PCR as compared to conventional protocols, in spite of its evidently high analytical sensitivity. On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings. (AU). Descriptors: Antígenos de Bactérias/GE Proteínas de Bactérias/GE Biópsia DNA Bacteriano/CH/IP Hanseníase/*DI/MI Dados de Sequência Molecular Mycobacterium leprae/GE/*IP Reação em Cadeia da Polimerase/*MT Sensibilidade e Especificidade Alinhamento de Sequência Análise de Sequência de DNA Pele/MI Uganda Limits: Research Support, Non-U.S. Gov't Humanos Location: BR191.1; 09344/s

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Id: 17563 Author: Zhang, Liangfen; Budiawan, Teky; Matsuoka, Masanori Title: Diversity of potential short tandem repeats in Mycobacterium leprae and application for molecular typing ..- Source: s.l; s.n; Oct. 2005. 9 p. tab. Abstract: A recent advance in molecular typing for tracing the transmission of leprosy is the discovery of short tandem repeats (STRs) in Mycobacterium leprae. To substantiate polymorphic loci from STR as promising candidates for molecular typing tools in leprosy epidemiology, 44 STR loci including 33 microsatellites and 11 minisatellites were investigated among 27 laboratory strains by sequencing PCR products. Not all STRs were necessarily polymorphic. Thirty-two out of the 44 loci were polymorphic. Nine polymorphic loci were suitable for identifying genotypes according to the discriminatory capacity, stability, and reproducibility. All the strains were classified into independent genotypes by the selected nine loci. Three multi-case households were subjected to molecular typing. M. leprae obtained from household cases showed identical copy numbers by TTC triplet alone, but the isolates from one family contact case were divided into different genotypes by adding eight other polymorphic loci. The combination of information from multiple loci allows increasing levels of discrimination and it is likely that the generation and documentation of data will result in the choice of a potential molecular typing tool for leprosy epidemiology. (AU). Descriptors: Técnica de Tipagem Bacteriana/* DNA Bacteriano/AN Pé/MI Marcadores Genéticos/*GE Hanseníase/EP/MI Camundongos Endogâmicos BALB C Camundongos Nus Repetições de Microssatélites/GE Repetições Mini-Satélites/GE Mycobacterium leprae/*CL/*GE Análise de Sequência de DNA Sequências Repetidas em Tandem/*GE Variação (Genética)/* Limits: Research Support, Non-U.S. Gov't Camundongos Humanos Animais Location: BR191.1; 09342/s

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Id: 17560 Author: Honore, N Title: Le genome de Mycobacterium leprae: de l'analyse de la sequence aux enjeux therapeutiques The Mycobacterium leprae genome: from sequence analysis to therapeutic implications- Source: s.l; s.n; 2002. 7 p. ilus, tab. Abstract: The genome of Mycobacterium leprae, the causative agent of leprosy, was analyzed by rapid sequencing of cosmids and plasmids prepared from DNA isolated from one patient's strain. Results showed that the bacillus possesses a single circular chromosome that differs from other known mycobacterium chromosomes with regard to size (3.2 Mb) and G + C content (57.8%). Computer analysis demonstrated that only half of the sequence contains protein-coding genes. The other half contains pseudogenes and non-coding sequences. These findings indicate that M. leprae has undergone a major reductive evolution leaving a minimal set of functional genes for survival. Study of the coding region of the sequence provides evidence accounting for the particular pathogenic properties of M. leprae which is an obligate intracellular parasite. Disappearance of numerous enzymatic pathways in comparison with M. tuberculosis, an intracellular pathogen comparable to M. leprae, could explain the differences observed between the two organisms. Genomic analysis of the leprosy bacillus also provided insight into the molecular basis for resistance to various antibiotics and allowed identification of several potential targets for new drug treatments. (AU). Descriptors: Cromossomos Bacterianos/*GE Cosmídeos/GE DNA Bacteriano/*AN Genoma Hanseníase/DT/*PP Mycobacterium leprae/*GE/*PY/PH Plasmídeos Research Support, Non-U.S. Gov't Análise de Sequência de DNA/* Limits: Humanos Location: BR191.1; 09339/s

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Id: 17278 Author: Kim, Se-Kom; Lee, Seong-Beom; Kang, Tae-Jin; Chae, Gue-Tae Title: Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR ..- Source: s.l; s.n; 2003. 6 p. ilus, tab. Abstract: The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU). Descriptors: DNA Bacteriano Resistência Bacteriana a Múltiplas Drogas Hansenostáticos Hanseníase Mycobacterium leprae Reação em Cadeia da Polimerase Polimorfismo (Genética) Polimorfismo Conformacional de Simples Fita Análise de Sequência de DNA Limits: Humano Coréia (geográfico) Mutação Puntual Location: BR191.1; 09178/s

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Id: 13953 Author: Kim, Se -Kon; Lee, Seong- Beom; Kang, Tae -Jin; Chae, Gue -Tae Title: Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR ..- Source: s.l; s.n; 2003. 6 p. ilus, tab. Abstract: The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU). Descriptors: DNA BACTERIANO/quim DNA BACTERIANO/genet Resistência Bacteriana a Múltiplas Drogas/genet COREIA (GEOGRAFICO) HANSENOSTATICOS/farmacol HANSENIASE/quimioter HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MUTACAO PUNTUAL REACAO EM CADEIA DA POLIMERASE/métodos POLIMORFISMO (GENETICA) POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUÊNCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 09096/s

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Id: 13931 Author: Honore, N Title: Le genome de Mycobacterium leprae: de l'analyse de la sequence aux enjeux therapeutiques The Mycobacterium leprae genome: from sequence analysis to therapeutic implications- Source: s.l; s.n; 2002. 7 p. ilus, tab. Abstract: L'analyse génomique de Mycobacterium leprae, l'agent pathogène de la lèpre, a été réalisée grâce au séquençage à haut débit des cosmides et plasmides préparés à partir de l'ADN d´une souche isolée d'un patient. Elle a montré que ce bacille possède un seul chromosome circulaire, dont la taille (3,2Mb) et la composition en G+C (57,8%) sont réduits par rapport aux austres chromosomes mycobactériens connus. L'analyse informatique de la séquence a mis en évidence que seule la moitié de la séquences est codante. L'autre moitié contient des pseudogènes et des séquences non codantes. Le génome de M. leprae a donc subi une évolution réductive très importante qui ne lui a laissé qu'un set minimun de gènes fonctionnels pour vivre. Les informations déduites de la partie codante de la séquence permettent d'appréhender les propriétés biologiques particulières de cet agent infectieux à développement intra-cellulaire obligatoire. De plus, la disparition de nombreuses voies enzymatiques par rapport à M. tuberculosis, autre pathogène intra-cellulaire en certains points semblable à M. leprae, explique les différences observées entre les deux organismes. Enfin, l'analyse génomique du bacille lépreux permet de comprendre les bases moléculaires de sa résistance à différents antibiotiques et d'identifier des cibles potentielles pour la mise au point de nouveaux traitements. (AU). Descriptors: DNA BACTERIANO/anal CROMOSSOMOS BACTERIANOS/genet COSMIDIOS/genet HANSENIASE/quimioter HANSENIASE/fisiopatol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/fisiol MYCOBACTERIUM LEPRAE/patogen GENOMA PLASMIDEOS ANALISE DE SEQUÊNCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T RESUMO EM INGLES Location: BR191.1; 09108/s

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Id: 13722 Author: Maeda, Yumi; Makino, Masahiko; Crick, Dean C; Mahapatra, Sebabrata; Srisungnam, Sopa; Takii, Takemasa; Kashiwabara, Yoshiko; Brennan, Patrick J Title: Novel 33-kilodalton lipoprotein from Mycobacterium leprae ..- Source: s.l; s.n; 2002. 6 p. tab, graf. Abstract: A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipoprotein was a membrane protein of M. leprae. The purified lipoprotein was found to induce production of interleukin-12 in human peripheral blood monocytes. The studies imply that M. leprae LpK is involved in protective immunity against leprosy and may be a candidate for vaccine design. (AU). Descriptors: SEQUENCIA DE AMINOACIDOS ANTIGENOS DE BACTERIAS/GE/*IM/IP PROTEINAS DE BACTERIAS/GE/*IM/IP CLONAGEM MOLECULAR ESCHERICHIA COLI EXPRESSAO GENICA GENES BACTERIANOS INTERLEUCINA-12/*BI LIPOPROTEINAS/GE/*IM/IP PROTEINAS DE MEMBRANA/GE/*IM/IP DADOS DE SEQUENCIA MOLECULAR PESO MOLECULAR MONOCITOS/IM MYCOBACTERIUM LEPRAE/GE/*IM/IP PRECURSORES DE PROTEINAS/GE/*IM/IP ANALISE DE SEQUENCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T SUPPORT, U.S. GOV'T, P.H.S. Location: BR191.1; 09059/s

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Id: 12298 Author: Dequeker, Jan; Fabry, Guy; Vanopdenbosch, Ludo Title: Hieronymus Bosch (1450-1516): paleopathology of the medieval disabled and its relation to the Bone and Joint Decade 2000-2010 ..- Source: s.l; s.n; Nov. 2001. 8 p. ilus, tab. Descriptors: DAPSONA DIIDROPTEROATO SINTASE RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUENCIA DE DNA Limits: ANIMAL Location: BR191.1; 08668/s

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Id: 12256 Author: Lee, S. B; Kim, S. K; Kang, T. J; Chae, G. T; Chun, J. H; Shin, H. K; Kim, J. P; Ko, Y. H; Kim, N. H Title: The prevalence of folP1 mutations associated with clinical resistance to dapsone, in mycobacterium leprae isolates from South Korea ..- Source: s.l; s.n; 2001. 4 p. ilus, tab. Descriptors: DAPSONA DIIDROPTEROATO SINTASE RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUENCIA DE DNA Limits: ANIMAL Location: BR191.1; 08670/s

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Id: 12248 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; Jun. 2001. 6 p. ilus, tab. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA Análise Heteroduplex/*MT Location: BR191.1; 08678/s; BR191.1; 08430/s

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Id: 12123 Author: Cole, S. T; Eigimeier, K; Parkhill, J; James, K. D; Thomson, N. R; Wheeler, P. R; Honore, N; Garnier, T; Churcher, C; Harris, D; Mungall, K; Basham, D; Brown, D; Chillingworth, T; Connor, R; Davies, R. M; Devlin, K; Duthoy, S; Feltwell, T; Fraser, A; Hamlin, N; Holroyd, S; Hornsby, T; Jagels, K; Lacroix, C; Maclean, J; Moule, S; Murphy, L; Oliver, K; Quail, M. A; Rajandream, M.-A; Rutherford, K. M; Rutter, S; Seeger, K; Simon, S; Simmonds, M; Skelton, J; Squares, S; Stevens, K; Taylor, K; Whitehead, S; Woodward, J. R; Barrell, B. G Title: Massive gene decay in the leprosy bacillus ..- Source: s.l; s.n; feb. 2001. 5 p. ilus, tab, graf. Descriptors: TATUS DNA BACTERIANO METABOLISMO ENERGÉTICO EVOLUÇAO MOLECULAR GENOMA BACTERIANO HANSENIASE DADOS DE SEQUENCIA MOLECULAR GENES REITERADOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE ANALISE DE SEQUENCIA DE DNA Transferência Genética Horizontal Limits: ANIMAL Location: BR191.1; 08699/s

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Id: 11752 Author: Mempel, Martin; Flageut, Beatrice; Suarez, Felipe; Ronet, Catherine; Dubertret, Louis; Korisky, Philippe; Gachelin, Gabriel; Musette, Philippe Title: Comparasion of the T cell patterns in leprous and cutaneous sarcoid granulomas. Presence of Valpha24-invariant natural killer T cells in T-cell-reactive leprosy together with a highly biased t cell receptor Valpha repertoire ..- Source: s.l; s.n; 2000. 16 p. ilus, tab, graf. Descriptors: HANSENIASE DERMATOPATIAS DERMATOPATIAS DERMATOPATIAS PELE PELE PELE SEQUENCIA DE AMINOACIDOS BIOPSIA DNA DNA GRANULOMA GRANULOMA GRANULOMA IMUNOHISTOQUIMICA CÉLULAS KILLER NATURAIS CÉLULAS KILLER NATURAIS DADOS DE SEQUENCIA MOLECULAR RECEPTORES PARA ANTIGENOS DE CÉLULAS T ALFA-BETA SARCOIDOSE SARCOIDOSE SARCOIDOSE ANALISE DE SEQUENCIA DE DNA LINFOCITOS T Location: BR191.1; 08522/s

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Id: 11682 Author: Ramasoota, Pongrama; Wongwit, Waranya; Sampunachot, Prasert; Unnarat, Komes; Ngamying, Maeya; Svenson, Stefan B Title: Multiple mutations in the rpoB gene of Mycobacterium leprae strains from leprosy patients in Thailand ..- Source: s.l; s.n; 2000. 5 p. tab. Descriptors: HANSENIASE HANSENIASE LEPROSTATICOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE CODON DNA BACTERIANO MUTAÇAO PROTEINAS DE PLANTAS REAÇAO EM CADEIA POR POLIMERASE RIFAMPINA ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 08474/s

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Id: 11681 Author: Rambukkana, Anura Title: M. leprae genome sequence ..- Source: s.l; s.n; 2001. 1 p. . Descriptors: HANSENIASE MYCOBACTERIUM LEPRAE SEQUENCIA DE BASES GENOMA BACTERIANO ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 08473/s

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Id: 11607 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; 2001. 6 p. tab, graf. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA ANALISE HETERODUPLEX/*MT Location: BR191.1; 08430/s

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Id: 11564 Author: Lee, S. B; Kim, S. K; Kang, T. J; Chee, G. T; Chun, J. H; Shin, H. K; Kim, J.P; Ko, Y. H Title: The prevalence of folP1 mutations associated with clinical resistance to dapsone, in Mycobacterium leprae isolates from South Korea ..- Source: s.l; s.n; 2001. 4 p. ilus, tab. Descriptors: DAPSONA DIIDROPTEROATO SINTASE RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUENCIA DE DNA Limits: ANIMAL Location: BR191.1; 08404/s

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Id: 11438 Author: Cole, S. T; Eigimeier, K; Parkhill, J; James, K. D; Thomson, N. R; Wheeler, P. R; Honoré, N; Garnier, T; Churcher, C; Harris, D; Mungall, K; Basham, D; Brown, D; Chillingworth, T; Connor, R; Davies, R. M; Devlin, K; Duthoy, S; Feltwell, T; Fraser, A; Hamlin, N; Holroyd, S; Hornsby, T; Jagels, K; Lacroix, C; Maclean, J; Moule, S; Murphy, L; Oliver, K; Quail, M. A; Rajandream, M.-A; Rutherford, K. M; Rutter, S; Seeger, K; Simon, S; Simmonds, M; Skelton, J; Squares, R; Stevens, K; Taylor, K; Whitehead, S; Woodward, J. R; Barrell, B. G Title: Masssive gene decay in the leprosy bacillus ..- Source: s.l; s.n; 2001. 5 p. ilus, tab, graf. Descriptors: TATUS METABOLISMO ENERGÉTICO DNA BACTERIANO EVOLUÇAO MOLECULAR GENOMA BACTERIANO HANSENIASE DADOS DE SEQUENCIA MOLECULAR GENES REITERADOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE ANALISE DE SEQUENCIA DE DNA Transferência Genética Horizontal Location: BR191.1; 08320/s

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Id: 10965 Author: El-Zaatari, Fouad A. K; Naser, Saleh A; Hulten, Kristina; Burch, Paula; Graham, David Y Title: Characterization of mycobacterium paratuberculosis p36 antigen and its seroreactivities in Crohn's diseases ..- Source: s.l; s.n; 1999. 5 p. ilus, tab. Descriptors: HANSENIASE MYCOBACTERIUM PARATUBERCULOSIS ANTICORPOS ANTIBACTÉRIAS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS DOENÇA DE CROHN DNA BACTERIANO ELETROFORESE EM GEL DE POLIACRILAMIDA IMMUNOBLOTTING PROTEINAS RECOMBINANTES ANALISE DE SEQUENCIA DE DNA TUBERCULOSE Location: BR191.1; 07428/s

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Id: 10315 Author: Haas, Christian J; Zink, Albert; Palfi, Gyorgy; Szeimies, Ulrike; Nerlich, Andreas G Title: Detection of leprosy in ancient human skeletal remains by molecular identification of Mycobacterium leprae ..- Source: s.l; s.n; 2000. 9 p. ilus, graf. Descriptors: SEQUENCIA DE BASES OSSO E OSSOS DNA BACTERIANO HISTORIA DA MEDICINA MEDIEVAL HISTORIA DA MEDICINA MODERNA HANSENIASE HANSENIASE HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE PALEONTOLOGIA PEPTIDIOS CICLICOS REAÇAO EM CADEIA POR POLIMERASE ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 07384/s

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Id: 9931 Author: Sakai, Tetsuo; Matsuo, Eiichi; Wakizaka, Akira Title: A genomic study of the leproma-derived and cultivable Mycobacterium HI-75. The direct DNA sequencing of PCR product of 16S ribosomal RNA ..- Source: s.l; s.n; 1996. 6 p. tab. Descriptors: HANSENIASE LEPROMATOSA MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM SCROFULACEUM SEQUENCIA DE BASES DADOS DE SEQUENCIA MOLECULAR REAÇAO EM CADEIA POR POLIMERASE RNA BACTERIANO RNA RIBOSSOMICO 16S ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 06860/s

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